Researchers working as part of the University of Georgia’s Regenerative Bioscience Center have developed a new way to identify and sort stem cells that may one day allow clinicians to restore vision to people with damaged corneas using the patient’s own eye tissue. The UGA researchers published their findings in Biophysical Journal.The cornea is a transparent layer of tissue covering the front of the eye, and its health is maintained by a group of cells called limbal stem cells. When these cells are damaged by trauma or disease, the cornea loses its ability to self-repair.“Damage to the limbus, which is where the clear part of the eye meets the white part of the eye, can cause the cornea to break down very rapidly,” said James Lauderdale, the paper co-author and associate professor of cellular biology in UGA’s Franklin College of Arts and Sciences. “The only way to repair the cornea right now is to do a limbal cell transplant from donated tissue.”In their study, researchers used a new type of highly sensitive atomic force microscopy, or AFM, to analyze eye cell cultures. Created by Todd Sulchek, an associate professor of mechanical engineering at Georgia Tech, the technique allowed researchers to probe and exert force on individual cells to learn more about the cell’s overall health and its ability to turn into different types of mature cells.They found that limbal stem cells were softer and more pliable than other cells, meaning they could use this simple measure as a rapid and cost-effective way to identify cells from a patient’s own tissue that are suitable for transplantation.“Todd’s technology is unique in the tiniest and most sensitive detection to change,” said Lauderdale. “Just think about trying to gently dimple or prod the top of an individual cell without killing it; with conventional AFM it’s close to impossible.”Building on their findings related to cell softness, the research team also developed a microfluidic cell sorting device capable of filtering out specific cells from a tissue sample. With this device, the team can collect the patient’s own tissue, sort and culture the cells, and place them back into the patient – all in one day, said Lauderdale. It can take weeks to perform this task using conventional methods.The researchers are quick to caution that more tests must be done before this technique is used in human patients, but it may one day serve as a viable treatment for the more than 1 million Americans who lose their vision to damaged corneas every year. The group first started this research with the hope of helping children with aniridia, an inherited malformation of the eye that leads to breakdown of the cornea at an early age. Because aniridia affects only one in 60,000 children, few organizations are willing to commit the resources necessary to combat the disease, Lauderdale said.“Our first goal in working with such a rare disease was to help this small population of children, because we feel a close connection to all of them,” said Lauderdale, who has worked with aniridia patients for many years. “However, at the end of the day this technology could help hundreds of thousands of people, like the military, who are also interested in corneal damage, common in desert conditions.”Steven Stice, a Georgia Research Alliance Eminent Scholar, who plays an important role in fostering cross-interdisciplinary collaboration as director of the RBC, initially brought the researchers together and encouraged a seed grant application through the center for Regenerative Engineering and Medicine, or REM, a joint collaboration between Emory University, Georgia Tech and UGA.“A culture is developing around seed funding that is all about interdisciplinary collaboration, sharing of resources, and coming together to make things happen,” said Stice. “Government funding agencies place a high premium on combining skills and disciplines. We can no longer afford to work in an isolated laboratory using a singular approach.”The REM seed funding program is intended to stimulate new, unconventional collaborative research and requires equal partnership of faculty from two of the participating institutions.“We tend to get siloed experimentally,” says Lauderdale. “To a biologist like me, all cells are very different and all atomic force microscopes are the same. To an engineer like Todd it’s just the opposite.”The study, “Cellular Stiffness as a Novel Stemness Marker in the Corneal Limbus,” is available at www.cell.com/biophysj/fulltext/S0006-3495(16)30771-8. Funding was provided by an NIH NIGMS Biotechnology Training Grant on Cell and Tissue Engineering, the Knights Templar Eye Foundation, the Center for Regenerative Engineering and Medicine, the Sharon Stewart Aniridia Research Trust and the NSF CMMI division.
Sign up for our COVID-19 newsletter to stay up-to-date on the latest coronavirus news throughout New York Alligators discovered in the Peconic River last April. (DEC)A small alligator initially spotted near the Peconic River in Calverton five days ago is doing its best to evade its captors.The New York State Department of Environmental Conservation received its first notification regarding the gator on Sunday after witnesses spotted the reptile near the Connecticut Avenue boat launch, according to Aphrodite Montalvo, a DEC spokeswoman.A witness spotted the potentially four-foot long gator again on Wednesday and the DEC made visual confirmation a day later, she said. But the scaly gator is still at large.“They spent a few hours trying to catch it on Thursday and were unsuccessful,” Montalvo said, adding that DEC officers are continuing the search.Recent storms that have dropped about 8 inches of rain on LI in the last week have made the search even more difficult because the water level in the Peconic River has risen and the water is moving “quite quickly,” Montalvo added.Alligators are illegal in New York State unless permits are approved for educational purposes.DEC officers are searching for the gator in the same vicinity where four baby alligators were captured from the Peconic River in April, and six months after nine other gators were discovered in a span of six weeks.Last fall’s alligator problem prompted the DEC and Suffolk County SPCA to hold an illegal reptile amnesty day.DEC officials have said that people acquire gators and soon discover that it becomes difficult to care for them once they grow.The baby alligators are capable of surviving the summer months but can’t withstand Northeast winters.The area of the most recent gator sighting is currently closed off, but if anyone does spot one, they should call DEC officers at 631-444-0250, Montalvo said.
Three credit union-specific regulatory relief provisions, as well as more than a dozen other relief items that benefit credit unions, are contained in a bill introduced by Senate Banking Committee Chairman Richard Shelby (R-Ala.) Tuesday.The much-anticipated bill is expected to be marked up by the Senate Banking Committee on May 21.“Several Title I provisions within Chairman Shelby’s draft legislation align with regulatory relief changes that CUNA has long advocated for on behalf of our members,” said CUNA President/CEO Jim Nussle when the bill was unveiled. “CUNA has repeatedly called on Congress to provide regulatory relief, and I thank Chairman Shelby and his staff for the many Title I provisions that will benefit credit unions and their members.”The credit union-specific provisions in the bill are:Allowing privately insured credit unions to become members of the Federal Home Loan Bank (FHLB) system;Granting credit unions under $1 billion in assets parity with like-sized banks by allowing less restrictive access to the FHLB system; and continue reading » ShareShareSharePrintMailGooglePinterestDiggRedditStumbleuponDeliciousBufferTumblr
The Premier County travel to Tralee to take on the Kingdom for the provincial quarter final as defending champions. Manager Tommy Toomey has named an experienced side for tonight’s clash….It comprises of 6 senior players among the starting 15, led by Captain Jimmy Feehan. The other senior stars are Colm O’Shaughnessy, Ross Mulcahy, Kevin Fahey, Liam Casey, and Josh Keane. In all there are 9 of last year’s panel also in the starting 15, 8 of which played competitively in last year’s championship.Outside of that, 5 of last year’s minor team, Danny Owens, Emmet Moloney, Jack Kennedy, Stephen Quirke and Tommy Nolan step up to the mark at U21 level while arriving new to the starting team is Shane O’Connell from Golden-Kilfeacle.Manager Tommy Toomey says although preparations haven’t been ideal for his side, he’s confident the players will be ready come throw-in time.The game throws in at Austin Stack Park at 7.30. Full Tipp team below: 1. Jake McDonald – Clonmel Commercials 2. Colm O’Shaughnessy – Ardfinnan 3. Jimmy Feehan (Capt.)- Killenaule 4. Danny Owens – Moyle Rovers 5. Ross Mulcahy – Moyle Rovers 6. Kevin Fahey – Clonmel Commercials 7. Shane O’Connell – Golden-Kilfeacle 8. Paul Shanahan – Upperchurch-Drombane 9. Liam Casey – Cahir10. Emmet Moloney – Drom & Inch11. Jack Kennedy – Clonmel Commercials12. Josh Keane – Golden-Kilfeacle13. Paul Maher – Kilsheelan-Kilcash14. Stephen Quirke – Moyle Rovers15. Tommy Nolan – Drom & InchSubs:16. Charlie Manton -Fethard17. Luke Boland – Moyle Rovers18. Brendan Martin – Kilsheelan-Kilcash19. Morgan Irwin – Moyle Rovers20. Tadhg Fitzgerald – Moyle Rovers21. Niall McKenna – Cahir22. Gerry Cronin – Ardfinnan23. Mark Russell – Aherlow Gaels24. Joe Ryan – KilmurryManager – Tommy ToomeySelectors – Liam Kearns, Paul Fitzgerald, Shane Stapleton.