VANCOUVER, British Columbia — The moratorium is over. It’s time to ramp up the trade talk.With the NHL’s trade deadline set to expire in just two weeks, I’m putting an end to my three-week ban on trade questions this week, indulging your unquenchable thirst for deadline speculation.What are the Sharks looking for? Who will they use as trade bait? Will we see a goalie deal?Without any further ado, let’s open the mailbag: From @marleaufan1001: what is the best fix for the Sharks’ …
(Visited 17 times, 1 visits today)FacebookTwitterPinterestSave分享0 Scientists at MIT publishing in PNAS1 detected instances of alternative splicing in over 1,000 genes of stem cells. They also computed possible isoforms of mRNA transcriptions and found 80% of them in the cells. Not only that, the isoforms (alternatively spliced versions of exons from the same gene) appeared to be functional: “We find that alternative splicing can modify multiple components of signaling pathways important for stem cell function,” they say. In short, alternative splicing, in which exons from genes are recombined in different ways, expands the information content of the genome:We also analyze the distribution of splice variants across different classes of genes. We find that tissue-specific genes have a higher tendency to undergo alternative splicing than ubiquitously expressed genes. Furthermore, the patterns of alternative splicing are only weakly conserved between orthologous genes in human and mouse. Our studies reveal extensive modification of the stem cell molecular repertoire by alternative splicing and provide insights into its overall role as a mechanism of generating genomic diversity. (Emphasis added in all quotes.)They took note that “different mRNA isoforms from a single gene can often encode proteins with distinct, sometimes opposite functions.” In fact, they point to earlier research that said, “Numerous biological processes ranging from sex determination to apoptosis depend on the alternative splicing of specific genes.” Later, they said, “alternative splicing was found to extensively affect components of signaling pathways that are functional in stem cells, suggesting an important role of splice variations in self-renewal and differentiation.” Thus, their work adds to a growing body of research showing that “alternative splicing is a general mechanism to increase the coding capacity and diversity of the genome in metazoans.” What regulates how the exons are spliced? “Previous studies of individual genes have shown that splicing is coupled to transcription by protein-protein interactions between components of the transcription and splicing complexes.” Their work suggested that tissue-specific genes seem to undergo the most alternative splicing, and ubiquitously-expressed genes less so. They offered an “evolutionary argument” that tissue-specific genes could afford more experimentation: “ubiquitous transcripts responsible for crucial and general cellular processes have evolved not to be modified, whereas diversification is advantageous for tissue-specific gene products.” This hypothesis, they felt, was reinforced by the finding that “patterns were conserved for only 20% of the examined orthologous genes in the human and mouse species, despite the general conservation of their exon-intron boundaries.” This, they feel, could lead to rapid evolution of alternatively spliced exons, and subsequently to functional differences in otherwise analogous cell types between distant species.1Pritzker et al., “Diversification of stem cell molecular repertoire by alternative splicing,” Proceedings of the National Academy of Sciences USA, 10.1073/pnas.0502132102, published online before print September 23, 2005.These findings add to the growing realization that the genome contains much more embedded information than mere gene count would suggest (see 09/08/2005 entry). If the introns themselves (02/18/2005, 02/02/2005) transcribe into RNA regulatory elements, then nothing is wasted, and nothing is junk. If all parts of the system can be shown to produce function, it becomes harder to claim evolution built this tight ship. These authors’ weak attempt to produce an “evolutionary argument” did not demonstrate that any heritable, functional advantage derived from mistakes in alternative splicing, but only that it could have. Did they demonstrate an example of a new function arising from a mistake? No; they just expressed faith that randomness creates the possibility space for order. This is a doctrine of pantheism (a religion). On the other hand, the high degree of conservation found in ubiquitously-expressed genes and at intron-exon boundaries are anti-evolutionary observations. To argue evolution out of this data is to rely again on slippery homology vs. analogy arguments (see “Homology for Dummies,” 05/05/2004). Because such arguments depend on embedded evolutionary assumptions, they are inherently circular. It is just as logical to conclude that a common Designer built the system around two principles: (1) modular construction, wherein commonly-needed functions are coded similarly between different organisms, and (2) robustness, in which regulatory networks can maintain stability in changing environments. The design inference has the added advantage of an adequate cause for the high degree of information involved. Whatever geneticists continue to uncover about the particulars, the system works. Somehow, a human genome gives rise to a human, and a mouse genome gives rise to a mouse. Unless mutations disrupt the program, the mouse will have all the parts in the right places. It will be covered with the right kind of fur, have the right teeth in the right order, have feet and muscles and eyes and a brain and every organ necessary for its little life. The molecular processes may seem disorganized to us. We see that one gene can be alternatively spliced into several products, some which can produce opposite functions. How does the right one get selected at the right time it is needed? There are wonderful mysteries here that could be illuminated by a scientist looking for intelligent design. If the DNA is not the master controller of its own transcription, what is? What controls the spliceosome? (09/17/2004). Can protein-protein interactions really be responsible for regulating the splicing, or is there another layer of genetic information directing their interplay? What do all those short non-coding RNAs do? How can so many competing processes and such a multiplicity of molecules guarantee a working mouse at the end of the assembly line? We see only glimpses of how the plethora of processes at the molecular level leads invariably to the right result. There may be more information and more design operating than we can possibly imagine.
Johannesburg, 25 September 2013 – Infrastructure in South Africa was foremost on the agenda as a group of African journalists embarked on a media tour, hosted by Brand South Africa. The journalists work for various media organisations based in Nigeria, Zimbabwe, Namibia and South Africa.During the tour, journalists will be exposed to South Africa’s infrastructure through the motor industry value chain. Journalists will see the full spectrum of production to shipment and will visit sites in Pretoria, Johannesburg and Durban. The tour is also intended to profile and highlight the opportunities for intra-African trade and how South Africa supports this continental priority.Brand South Africa CEO Miller Matola said, “South Africa’s infrastructure gained momentum in the run up to the 2010 FIFA World Cup and has over the years expanded as the foundation of a national growth and development strategy. Our transport, water, electricity and telecommunications networks are being extended and we are thus strengthening the fabric of South African communities. Recent studies have also indicated that in terms of innovation, our country is performing strongly.”On Wednesday, the delegation travelled via the Gautrain from Johannesburg to Pretoria. There, they toured the Roslyn Nissan site, which is a key vehicle manufacturing and assembly plant. The plant is a leader in sound environmental practice with regards to vehicle manufacturing and has won numerous awards for its environmental efforts. This was followed by a visit to the Automotive Supplier Park in Tshwane, which is a Gauteng Provincial government Initiative aimed at stimulating economic growth and job creation in the automotive industry, through investment in strategic infrastructure.As part of the day’s activities, journalists also visited Freedom Park during which efforts to build the emotional, psychological and social capital of the country’s citizens through reconciliation and healing was showcased.The journalists are this week also scheduled to visit Johannesburg’s City Deep Port, Durban’s New Cargo Port and the Moses Mabhida Stadium, among other sites.About Brand South AfricaBrand South Africa is the official marketing agency of South Africa, with a mandate to build the country’s brand reputation, in order to improve its global competitiveness abroad. Its aim is also to build pride and patriotism among South Africans, in order to contribute to social cohesion and nation brand ambassadorship.Further resources from Brand South AfricaMedia are invited to visit http://www.southafrica.info/ for further resources which can be reproduced without any copyright infringement. Kindly attribute to Brand South Africa.For more information or to set up interviews, please contact:Nadia Samie-JacobsPublic Relations DomesticTel: +27 11 712 5007 Mobile: +27 (0)72 777 9399Email: email@example.comVisitwww.brandsouthafrica.comEnds
24 July 2015The South African Revenue Service (Sars) introduced a new, state-of-the-art container scanner at the Cape Town harbour on Wednesday.This is the second scanner of its kind in South Africa; the first one was installed in Durban. It will be used to clamp down on illegal trade, which robs the country of millions of rand in unpaid duties and taxes.“With the new high-tech scanners in Durban and Cape Town, Sars is doing end-to-end integrated cargo scanning for the first time,” the organisation said.“In other words, our risk engine, case management system and scanner software is now integrated into one solution that is automated and real-time, with the whole process recorded on the Sars system from beginning to end.”Game over for criminalsSars commissioner Tom Moyane told Fin24 that “we should deter those who would like to avoid paying their fair value in terms of customs. That game is over.”The scanner cost R38.5-million but Moyane said this was a worthwhile price. “You want to talk about return on investment? Let’s talk numbers: R986-billion is what we collected. Customs contributed approximately 9% to 10%.“Utilisation of the scanners is to deter and detect that which we would not have been able to bring into the fiscus. So if we used the scanners a year before, we could have collected close to a trillion (rand),” he explained.How it worksThe scanners use X-ray technology coupled with radiation scanning to detect up to 40 different materials, including aluminium, steel, plastic, and organic components, according to the technology news website, MyBroadband Business Tech.It is also able to scan through 380mm of solid steel, and cargo can be scanned in under 12 minutes. Sars aims to complete a hundred inspections a day.The radiation portal will also be able to tip off customs officials if radioactive material is being smuggled.SAinfo reporter
A Web Developer’s New Best Friend is the AI Wai… Related Posts Why Tech Companies Need Simpler Terms of Servic… Twenty-year PR veteran Sean Garrett just announced that he’s leaving the communications startup he helped found five years ago and taking a new position as vice president of communications at Twitter. Garrett says he’s been consulting for Twitter for the past few months, via 463 Communications. Twitter has been hiring fast lately, often picking up smart people working at partner companies. Garrett is a UCLA grad in Political Science and first worked in the office of California Governor Pete Wilson in 1990. He went on to do PR with many of the biggest tech companies in Silicon Valley.Garrett created an account on Twitter on March 8th, 2007 (co-incidentally, one day after I joined, so our anniversaries are coming soon, Sean!) and pays attention on Twitter to people like Mike Masnick of the tech blog TechDirt and maverick PR guy Jeremy Pepper.Twitter desperately needs communications help. The company has grown in importance faster than it’s been able to keep up with. Garrett’s phone will no doubt be ringing off the hook.He’ll be working in the Communications Department with Jenna Sampson, who came from Yahoo!, former CNN correspondent Alexa Lee and Emily Pinkerton, among others. You can follow all four on Twitter via this list. Top Reasons to Go With Managed WordPress Hosting Tags:#news#NYT#web marshall kirkpatrick 8 Best WordPress Hosting Solutions on the Market
The Northeast Frontier Railway (NFR) has declined to transport four elephants from eastern Assam’s Tinsukia to Ahmedabad for the July 4 Jagannath Rath Yatra until the Gauhati High Court resolves the matter and the State Forest Department clarifies an order of the Supreme Court regarding transfer of elephants.Assam-based animal rights activists Urmi Mala Das and Nandini Baruah had on Friday filed a public interest litigation at the high court challenging the Assam government’s decision to transport four juvenile elephants, two of them females, in railway wagons to Ahmedabad. Their PIL followed a petition filed via email to the court by Canada-based activist Sangeeta Iyer.PIL filed“We will not dispatch the elephants unless we receive a clarification sought from the Forest Division on an order by the Supreme Court. Besides, there is a PIL to watch out for,” said Masood Alom, the Additional Divisional Railway Manager of NFR’s Tinsukia Division. Hearing a case related to Kerala, the SC had in May 2016 said: “The state government shall not issue any ownership certificate to any of the persons in possession of elephants. That apart, the persons who are in possession of elephants shall not transfer the elephants outside the state nor shall they part with the elephants by way of transfer in any manner…” Officials of NRF’s zonal headquarters in Guwahati’s Maligaon confirmed the decision not to transport the elephants until the legal issues are resolved. They also trashed reports that the elephants were being dispatched quietly before the PIL is heard on Monday.
Mac Cardona hopes to make a return to the PBA and his potential comeback could also be a reunion of sorts.FEATURED STORIESSPORTSSEA Games: Biñan football stadium stands out in preparedness, completionSPORTSPrivate companies step in to help SEA Games hostingSPORTSWin or don’t eat: the Philippines’ poverty-driven, world-beating pool starsThe 35-year-old Cardona, who survived a pill overdose in an apparent suicide attempt nearly a year ago, is trying out for the GlobalPort Batang Pier, a team that is all to familiar with the veteran swingman.ADVERTISEMENT “We are looking for other options and we feel Cardona is in the equation,” GlobalPort head coach Franz Pumaren told INQUIRER.net in a text message Thursday when asked if Cardona is trying to make the team. FEU Auditorium’s 70th year celebrated with FEU Theater Guild’s ‘The Dreamweavers’ Robredo should’ve resigned as drug czar after lack of trust issue – Panelo Pumaren cleared Cardona is not yet part of the team and that he has to prove that he can still play at a high level.“We still have to take a look at him in the next few days if he is still capable of playing in the PBA,” Pumaren said.Cardona last played in the PBA with the NLEX Road Warriors in 2016 until he was let go by the team before the start of the 42nd season after a series of misfortunes that included an arrest due to domestic violence and sleeping pill overdose that almost ended his life in August of last year.Cardona saw action in the PBA D-League as a member of Zark’s Burgers.Sports Related Videospowered by AdSparcRead Next Ethel Booba on hotel’s clarification that ‘kikiam’ is ‘chicken sausage’: ‘Kung di pa pansinin, baka isipin nila ok lang’ READ: Mac Cardona to suit up for Zark’s BurgersCardona created a stir on social media after posting a photo of himself joining the Batang Pier in one of their practice sessions at Gatorade Hoops Center in Mandaluyong City.In the Instagram photo, Cardona is with former fellow La Salle alums Mike Cortez, Ryan Araña and Rico Maierhofer, Renren Ritualo and Arnold Van Opstal and Pumaren, who was his coach with the Green Archers.Cortez and Araña were teammates with Cardona during their collegiate days.READ: Cardona takes another step in rebuilding his lifeADVERTISEMENT National Coffee Research Development and Extension Center brews the 2nd National Coffee Education Congress View comments Trump strips away truth with hunky topless photo tweet Don’t miss out on the latest news and information. MOST READ Lacson: SEA Games fund put in foundation like ‘Napoles case’ Robredo: True leaders perform well despite having ‘uninspiring’ boss PLAY LIST 02:49Robredo: True leaders perform well despite having ‘uninspiring’ boss02:42PH underwater hockey team aims to make waves in SEA Games01:44Philippines marks anniversary of massacre with calls for justice01:19Fire erupts in Barangay Tatalon in Quezon City01:07Trump talks impeachment while meeting NCAA athletes02:49World-class track facilities installed at NCC for SEA Games #KicksStalker: Is a Filipino signature shoe on the horizon? LATEST STORIES DILG, PNP back suspension of classes during SEA Games Church, environmentalists ask DENR to revoke ECC of Quezon province coal plant
Anna University TANCET results on June 22.The results of TANCET 2011 (Tamil Nadu Common Entrance Test) organised by the Anna University will be declared on June 22.The TANCET results 2011 will be available at the website of Anna University www.results.annauniv.edu.Anna University organises the TANCET examinations on behalf of the Tamil Nadu government for admissions to MBA, MCA, MTech and ME courses in the state.For more news on India, click here.For more news on Business, click here.For more news on Movies, click here.For more news on Sports, click here.
Austrian sixth seed Dominic Thiem got his French Open campaign off to a quickfire start on Sunday, beating an out-of-sorts Bernard Tomic 6-4 6-0 6-2 as he embarked on a bid to go one round better than he did last year.After a tight first set, the 39th-ranked Australian won just two more games as, looking to keep the points short on a hot and fast Court Suzanne Lenglen, he sprayed the ball long and wide in his quest for winners.In all he made 33 unforced errors to Thiem’s 10, while the ace count was 11 to three in the Austrian’s favour.”I am happy I had a good start,” Thiem said courtside after the match, though in truth he might have preferred a tougher workout in keeping with his ambitions for the tournament.Speaking before Sunday’s match Thiem, a semi-finalist in 2016, put himself in the category of “players who can go very deep here (and) …make big surprises” behind clear favourite Rafa Nadal.The Austrian and the Spaniard have met three times so far in 2017, including the quarter-finals of the Rome Masters, where 23-year-old Thiem became the only player to beat Nadal on clay this season.DIMITROV GOES PAST ROBERTBulgarian tennis player Grigor Dimitrov eliminated French Stephane Robert from the French Open first round here on Sunday.Dimitrov defeated his French rival 6-2, 6-3, 6-4 to reach the second round of the tournament, reports Efe.The Bulgarian victor is to play the second round against the winner of the duel between Spanish Tommy Robredo and Briton Daniel Evans.advertisement(With inputs from IANS)